Innovation Center of Faculty of Chemistry, University of Belgrade , Belgrade , Serbia
Institute for Multidisciplinary Studies, University of Belgrade , Belgrade , Serbia
Institute for Multidisciplinary Studies, University of Belgrade , Belgrade , Serbia
Faculty of Chemistry, University of Belgrade , Belgrade , Serbia
Institute of Chemistry, Technology and Metallurgy, University of Belgrade , Belgrade , Serbia
Faculty of Chemistry, University of Belgrade , Belgrade , Serbia
Strategies based on the enzyme application are increasingly replacing the conventional chemical procedures because of their efficiency, quicker performance and environmental protection. However, natural enzymes can rarely be used in industry since their beneficial features can not endure the industrial conditions. Additional drawbacks of natural enzymes are their inhibition by reaction products and difficulty to be removed from the reaction mixture. The most promising technique to substantially improve the enzyme properties, such as activity, pH, thermal and organic-solvent stability, reusability and storage stability, in non-natural environments is by the enzyme immobilization. In this review we summarized different techniques used to immobilize enzymes to inert carriers. A wide variety of materials of both the organic and inorganic origin was used as carriers for the enzyme immobilization. We also summarized a class of new materials where the enzyme performance was enhanced by combining different classical materials and shaping in specific forms.
Authors retain copyright. This work is made freely available online under an open-access model under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC-http://creativecommons.org/licenses/by-nc-nd/4.0/BY-NC-ND 4.0).
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International Journal of Biological Macromolecules, 163()
10.1016/j.ijbiomac.2020.09.021
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10.1016/j.snb.2025.137460
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(2020)
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Bioresource Technology Reports, 12()
10.1016/j.biteb.2020.100602
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(2022)
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Science of The Total Environment, 806()
10.1016/j.scitotenv.2021.150500
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(2024)
Hyperactivation of crosslinked lipases in elastic hydroxyapatite microgel and their properties
Journal of Biological Engineering, 18(1)
10.1186/s13036-024-00440-5
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(2025)
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10.1021/acsomega.5c01613
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(2024)
Multi-walled carbon nanotubes as lipase carriers for organic synthesis: Current trends and recent update
Hemijska industrija, 78(1)
10.2298/HEMIND230614003P
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(2023)
Ligand fishing as a tool to screen natural products with anticancer potential
Journal of Separation Science, 46(12)
10.1002/jssc.202200964
Sarita Pathania, Aryan Jyoti, Aakash Rathour
(2025)
Enzyme Immobilization with Nanomaterials: Applications and Challenges
ACS Symposium Series, 1508()
10.1021/bk-2025-1508.ch002
Rita A. M. Barros, Loan Rivereau, Maria J. Sampaio, Raquel O. Cristóvão, Cláudia G. Silva, Joaquim L. Faria
(2025)
Graphitic Carbon Nitride: A Reusable and Stable Support for Glucose Oxidase
Langmuir, 41(25)
10.1021/acs.langmuir.5c00737
Aya A. Najim, Ahmed Y. Radeef, Ibrahim al‐Doori, Zaid H. Jabbar
(2024)
Immobilization: the promising technique to protect and increase the efficiency of microorganisms to remove contaminants
Journal of Chemical Technology & Biotechnology, 99(8)
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