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Original scientific paper

Characterisation and antimicrobial activity of silver nanoparticles derived from Vascellum pratense polysaccharide extract and sodium citrate

By
Predrag Petrović ,
Predrag Petrović
Contact Predrag Petrović

Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

Danijela Kostić ,
Danijela Kostić

Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

Anita Klaus ,
Anita Klaus

Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

Jovana Vunduk ,
Jovana Vunduk

Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

Miomir Nikšić ,
Miomir Nikšić

Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

Đorđe Veljović ,
Đorđe Veljović

Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

Leo van Griensven
Leo van Griensven

Plant Research International, Wageningen University & Research, Wageningen, Netherlands

Abstract

Silver nanoparticles (AgNPs) were synthesized by “green”, cheap hydrothermal method in an autoclave using sodium citrate and Vascellum pratense polysaccharide extract as reducing and stabilizing agents. Presence of spherical AgNPs was confirmed by UV-VIS spectrophotometry and scanning electron microscopy; particle size was determined as ~ 40 nm. Even though colloidal solution had relatively low absolute value of zeta potential(-15 mV), short term stability studies suggested a stable system, with AgNPs being stabilized by both citrate and fungal polysaccharides, as FTIR spectra confirmed. The colloidal solution showed good antimicrobial activity against both G+/G- bacteria and Candida albicans, including methicilin resistant Staphylococcus aureus (MRSA). Products containing AgNPs and fungal polysaccharides, which possess various biological activities - most important being immunostimulation - may find use in treatment of skin conditions caused by pathogens.

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Authors retain copyright. This work is made freely available online under an open-access model under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC-http://creativecommons.org/licenses/by-nc-nd/4.0/BY-NC-ND 4.0).

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