RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Abstract
The retention behaviour and separation ability of some estradiol derivatives were studied by HPLC on silica and C-18 commercially available columns. The mobile phases used were: benzene-ethyl acetate, benzene-tetrahydrofuran, benzene-acetonitrile, isopropanol-water and tetrahydrofuran-water in various proportions. The results are discussed in terms of nature of the solute, eluent and stationary phase. Correlation between the retention constants of estradiol derivatives obtained on C-18 column and log P calculated by program ACD log P was examined too.
Keywords: estradiol derivatives, chromatography, normal phases, reversed phases, retention, lipophilicity
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