RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Marijana Ačanski; Đura Vujić; Zvonko Nježić

Original scientific paper

RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

- Marijana Ačanski
- University of Novi Sad, Novi Sad, Faculty of Technology, Serbia
- Đura Vujić
- University of Novi Sad, Novi Sad, Faculty of Technology, Serbia
- Zvonko Nježić
- University of Novi Sad, Novi Sad, Institute of Food Technology, Serbia

Abstract

The retention behaviour and separation ability of some estradiol derivatives were studied by HPLC on silica and C-18 commercially available columns. The mobile phases used were: benzene-ethyl acetate, benzene-tetrahydrofuran, benzene-acetonitrile, isopropanol-water and tetrahydrofuran-water in various proportions. The results are discussed in terms of nature of the solute, eluent and stationary phase. Correlation between the retention constants of estradiol derivatives obtained on C-18 column and log P calculated by program ACD log P was examined too.

Keywords: estradiol derivatives, chromatography, normal phases, reversed phases, retention, lipophilicity

How to Cite: Ačanski, M. , Vujić, Đ. & Nježić, Z. (2012) “RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY”, Journal of Engineering & Processing Management. 4(1).