Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
Institute of Food Technology, University of Novi Sad, Novi Sad, Serbia
The retention behaviour and separation ability of some estradiol derivatives were studied by HPLC on silica and C-18 commercially available columns. The mobile phases used were: benzene-ethyl acetate, benzene-tetrahydrofuran, benzene-acetonitrile, isopropanol-water and tetrahydrofuran-water in various proportions. The results are discussed in terms of nature of the solute, eluent and stationary phase. Correlation between the retention constants of estradiol derivatives obtained on C-18 column and log P calculated by program ACD log P was examined too.
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