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Original scientific paper

RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

By
Marijana Ačanski ,
Marijana Ačanski
Contact Marijana Ačanski

Faculty of Technology, University of Novi Sad, Novi Sad, Serbia

Đura Vujić ,
Đura Vujić

Faculty of Technology, University of Novi Sad, Novi Sad, Serbia

Zvonko Nježić
Zvonko Nježić

Institute of Food Technology, University of Novi Sad, Novi Sad, Serbia

Abstract

The retention behaviour and separation ability of some estradiol derivatives were studied by HPLC on silica and C-18 commercially available columns. The mobile phases used were: benzene-ethyl acetate, benzene-tetrahydrofuran, benzene-acetonitrile, isopropanol-water and tetrahydrofuran-water in various proportions. The results are discussed in terms of nature of the solute, eluent and stationary phase. Correlation between the retention constants of estradiol derivatives obtained on C-18 column and log P calculated by program ACD log P was examined too.

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Authors retain copyright. This work is made freely available online under an open-access model under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC-http://creativecommons.org/licenses/by-nc-nd/4.0/BY-NC-ND 4.0).

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