The Enzyme Immobilization: Carriers and immobilization methods

  • Milica Spasojevic Innovation Center of Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia
  • Olivera Prodanovic Institute for Multidisciplinary Studies, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia
  • Nevena Pantic Institute for Multidisciplinary Studies, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia
  • Nikolina Popovic University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, Belgrade, Serbia
  • Ana Marija Balaz Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 2, 11000 Belgrade, Serbia
  • Radivoje Prodanovic University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, Belgrade, Serbia

Abstract

Strategies based on the enzyme application are increasingly replacing the conventional chemical procedures because of their efficiency, quicker performance and environmental protection. However, natural enzymes can rarely be used in industry since their beneficial features can not endure the industrial conditions. Additional drawbacks of natural enzymes are their inhibition by reaction products and difficulty to be removed from the reaction mixture. The most promising technique to substantially improve the enzyme properties, such as activity, pH, thermal and organic-solvent stability, reusability and storage stability, in non-natural environments is by the enzyme immobilization. In this review we summarized different techniques used to immobilize enzymes to inert carriers. A wide variety of materials of both the organic and inorganic origin was used as carriers for the enzyme immobilization. We also summarized a class of new materials where the enzyme performance was enhanced by combining different classical materials and shaping in specific forms.


Keywords: enzymes, immobilization, macroporous, carriers, hydrogels.

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Published
2020-03-12
How to Cite
SPASOJEVIC, Milica et al. The Enzyme Immobilization: Carriers and immobilization methods. Journal of Engineering & Processing Management, [S.l.], v. 11, n. 2, p. 89-105, mar. 2020. ISSN 2566-3615. Available at: <https://jepm.tfzv.ues.rs.ba/index.php/Journal/article/view/202>. Date accessed: 07 july 2020. doi: https://doi.org/10.7251/JEPM1902089S.